Capillary Ion Analyzer – analysis of organic acids in small samples (Organic Acids).
Capillary Electrophoresis (CE) allows for the analysis of ions of (in-)organic metabolites. Samples can be dental plaque, tongue coating, saliva, in-vitro biofilms. This enables us to measure possible effects of oral care treatments on the capacity of biofilms to form organic acids at sub-millimolar levels after a sugar pulse. Measurements are performed on a Beckman P/ACE MDQ system, using UV detection.
Example
See this example of an acid profile in aqueous extract of dental buccal plaque (.jpg). Ions that can be analysed: Oxalate, formate, succinate, acetate, lactate, phosphate, propionate, butyrate, nitrate, nitrite.
References
- Gerardu, V.A., et al., Effects of various rinsing protocols after the use of amine fluoride/stannous fluoride toothpaste on the acid production of dental plaque and tongue flora. Caries Res, 2006. 40(3): p. 245-50.
- Koopman, J.E., et al., Nitrate and the Origin of Saliva Influence Composition and Short Chain Fatty Acid Production of Oral Microcosms. Microb Ecol, 2016. 72(2): p. 479-92.
More information
For more information: m.buijs@acta.nl