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Gas Liquid Chromatography -  Fluoride analysis

Fluoride Analysis (Ion Selective Electrode – Gas Liquid Chromatography).

Fluoride is important for caries prevention. Fluoride levels can be analyzed using two techniques, Ion Selective Electrode (ISE) and Gas Liquid Chromatography (GLC).

The ISE allows for the analysis of available F in a buffered system. The GLC measures fluoride after sample destruction in strong acid followed by derivatization to a volatile component

With these techniques it is possible to measure all common fluoride components (NaF, MFP, AmF, SnF2, TiF4) in a wide variety of products and carriers. Typical applications are; total and available fluoride in Oral Care products (Toothpaste,Rinse solutions), fluoride in saliva/plaque, loosely bound/incorporated fluoride in enamel and dentin.

References

  • Van Loveren, C., et al., Total and free fluoride in toothpastes from some non-established market economy countries. Caries Res, 2005. 39(3): p. 224-30.
  • Heijnsbroek, M., et al., Increased salivary fluoride concentrations after post-brush fluoride rinsing not reflected in dental plaque. Caries Res, 2006. 40(5): p. 444-8.
  • Van Strijp, A.A., M.J. Buijs, and J.M. ten Cate, In situ fluoride retention in enamel and dentine after the use of an amine fluoride dentifrice and amine fluoride/sodium fluoride mouthrinse. Caries Res, 1999. 33(1): p. 61-5.

More information

Contact Mark Buijs through m.Buijs@acta.nl.

Preventive Dentistry: Research on dental hard tissues